NextSeq 500 System

The First High-Throughput Desktop Sequencer

The NextSeq 500 Sequencing System delivers the power of high-throughput sequencing with the speed, simplicity, and affordability of a desktop NGS (next-generation sequencing) system. The fast, integrated, sample-to-results workflow enables many sequencing applications—including exomes, whole genomes, and transcriptomes—in a single run. This NGS system fits seamlessly into research laboratories, with no need for specialized equipment. Now, researchers can perform any combination of high- and mid-throughput sequencing applications to advance their studies.

Flexible and Scalable NGS System for Any Application

With the NextSeq 500 System, researchers can perform multiple sequencing applications on one platform. The NextSeq 500 System is the only NGS system capable of sequencing a whole human genome at high coverage (30×) in one run. It also delivers one-day turnaround for a number of popular sequencing applications. Researchers can switch between two configurations at the push of a button to perform mid-throughput sequencing applications or smaller sample batch sizes when needed.
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Fast and Easy Workflow

Designed to reduce setup time, the NextSeq 500 System features a load-and-go workflow and an intuitive user interface. Users can simply load flow cells and reagent cartridges and then sequence at the touch of a button. Without requiring additional equipment, the NextSeq 500 System integrates cluster generation, sequencing by synthesis, and base calling into a single NGS system.

Researchers can monitor run performance on the instrument or from any location as the run progresses. To streamline run management, downstream analysis, and data sharing, NextSeq 500 Systems offer the option to connect to BaseSpace, the Illumina genomic computing environment that is available in both cloud and on-site packages.
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Proven Illumina Technology

The NextSeq 500 System uses the same proven reversible-terminator sequencing by synthesis (SBS) technology used by all Illumina NGS systems, with recent innovations reducing cycle and data processing times. SBS technology is the most successful and widely adopted NGS technology worldwide.
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Simplified Informatics

The Illumina informatics solution enables researchers to go from sample to publication without bioinformatics expertise. Designed to minimize setup time and the risk of user error, the NextSeq 500 System's software enables researchers to review real-time data and performance metrics from anywhere at any time. Available in both cloud and on-site environments, the optional BaseSpace solution provides an easy, cost-effective way to prepare runs, track samples, and store, analyze, and share genomic data.
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End-to-End Scientific Support

Illumina technical support begins when the NextSeq 500 System is delivered, with Illumina scientists and engineers assisting with NGS system installation and setup, and the training of laboratory personnel. In addition to on-site support, training courses are available to bring laboratory personnel quickly up to speed. Illumina scientists are available 24/7 globally to answer questions every step of the way, enabling researchers to focus on making the next breakthrough discovery.
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NextSeq 500 Sequencer