MiSeq Kits

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MiSeq Reagent Kit v3

 

MiSeq Reagent Kits v3 enable the highest output of all MiSeq kits. MiSeq Reagent Kits v3 retain the same pre-filled, ready-to-use reagent cartridges as the v1 and v2 kits, but offer improved chemistry to increase cluster density and read length as well as improve quality (Q) scores. Updated system software unlocks the full benefits of v3 kits. Download Software »

 

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MiSeq Reagent Kit v3
 
MS-102-3001
 
 
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TruSeq Stranded mRNA Sample Prep Kit

 

The TruSeq Stranded mRNA Sample Preparation Kit provides the clearest and most complete view of the transcriptome with a streamlined, cost-efficient and scalable solution for mRNA analysis.

 

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TruSeq Stranded mRNA Sample Prep Kit
 
RS-122-2101
 
 
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MiSeq Reagent Kits v2

 

MiSeq Reagent Kits v2 enable more output to simplify each MiSeq run. MiSeq Reagent Kits v2 retain the same pre-filled, ready-to-use reagent cartridges as the v1 kits, but offer improved chemistry to increase cluster density, decrease cycle time, and improve quality (Q) scores. MiSeq Reagent Kits v2 are also available in micro and nano formats for low output applications.

 

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MiSeq Reagent Kits v2
 
MS-102-2001
 
 
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TruSeq Small RNA Sample Preparation Kit

 

TruSeq Small RNA Sample Preparation Kits provide a simple, cost-effective solution for generating small RNA libraries directly from total RNA. MicroRNAs (miRNAs) generated by Dicer processing are efficiently targeted by the included modified adapters. These kits enable multiplexed sequencing with the introduction of 48 unique indexes, allowing microRNA discovery and profiling throughput to match Illumina's unparalleled sequencing output. Indexes are added in a universal amplification reaction, greatly reducing ligation bias and ensuring accurate measurement of miRNA expression.

 

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TruSeq Small RNA Sample Preparation Kit
 
RS-200-0012
 
 
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Nextera XT DNA Sample Preparation Kit

The Nextera XT DNA Sample Preparation Kit allows the preparation of sequencing-ready libraries for small genomes (bacteria, archaea, viruses), amplicons, and plasmids in less than 90 minutes with only 15 minutes of hands-on time.

“We run samples from cancer and somatic genomes all using Nextera XT, all with excellent results, with a short time of hands-on work, and a very good quality of data.” —Lab Manager, Educational Institution

 

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Nextera XT DNA Sample Preparation Kit
 
FC-131-1024
 
 
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TruSeq Nano DNA Sample Prep Kit

 

The TruSeq Nano DNA Sample Preparation Kit enables efficient interrogation of samples with limited available DNA. Based on the industry’s most widely adopted sample preparation workflow, this low-input method delivers high coverage quality and reduced bias for virtually any sequencing application.

  • Designed for low sample input
  • High coverage quality
  • Accelerated sample preparation

 

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TruSeq Nano DNA Sample Prep Kit
 
FC-121-4001
 
 
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Nextera DNA Sample Preparation Kit

 

Nextera DNA Sample Preparation Kits provide the fastest and easiest workflow, enabling sequencing-ready libraries to be generated in less than 90 minutes, with less than 15 minutes of hands-on time. DNA is simultaneously fragmented and tagged with sequencing adapters in a single step, using standard lab equipment. Libraries prepared with Nextera kits are compatible with all Illumina sequencers.

 

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Nextera DNA Sample Preparation Kit
 
FC-121-1031
 
 
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TruSeq RNA Sample Preparation Kit v2

 

The TruSeq RNA Sample Preparation Kits provide a simple, cost-effective solution for generating mRNA-focused libraries from total RNA. The kits feature 24 unique indexes, delivering enhanced multiplex performance for processing up to 384 RNA-Seq samples on a single HiSeq 2500 run.

 

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TruSeq RNA Sample Preparation Kit v2
 
RS-122-2001
 
 
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TruSeq DNA PCR-Free Sample Preparation Kit

 

TruSeq DNA PCR-Free Sample Preparation Kits provide simple, all-inclusive sample preparation for whole-genome sequencing applications. Researchers can sequence a wide variety of organisms, from small genomes such as bacteria to whole-human genomes. The kits offer shortened gel-free workflows, the ability to sequence the most challenging regions, and the power to identify the greatest number of variants. Kits include reagents, sample purification beads, and indexes, with two options for flexibility:

  • TruSeq DNA PCR-Free LT Sample Preparation Kits for low-throughput studies. Includes 24 single indexes.
  • TruSeq DNA PCR-Free HT Sample Preparation Kits for high-throughput studies. Includes 96 dual indexes pre-loaded in a 96-well plate.

 

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TruSeq DNA PCR-Free Sample Preparation Kit
 
FC-121-3001
 
 
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TruSight One Sequencing Panel

 

Enabling labs to expand and streamline their sequencing portfolio, while managing costs.

  • Targets 4,813 genes associated with known clinical phenotypes
  • One workflow to follow and one procedure for managing genomic samples, increasing productivity, reducing handling errors, and decreasing laboratory costs

 

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TruSight One Sequencing Panel
 
FC-141-1006
 
 
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TruSight Autism

 

Assists in the investigation of genomic features associated with autism.

  • Developed in collaboration with Dr. Jonathan Pevsner and team at Kennedy Krieger Institute
  • Targets 101 developmental delay genes linked specifically to autism

 

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TruSight Autism
 
TG-141-1003
 
 
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TruSight Cancer

 

Targeting genes previously linked to a predisposition towards cancer.

  • Developed in collaboration with Professor Nazneen Rahman and team at the Institute of Cancer Research (ICR), London
  • Targets 94 genes and 284 SNPs associated with a predisposition towards cancer

 

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TruSight Cancer
 
TG-141-1002
 
 
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TruSight Cardiomyopathy

 

Focusing on identifying inherited causes of cardiomyopathy.

  • Developed in collaboration with Dr. Heidi Rehm, Dr. Birgit Funke, and their team at the Laboratory for Molecular Medicine (LMM) and Partners Healthcare Center for Personalized Genetic Medicine (PCPGM), Harvard Medical School
  • Targets 46 genes linked to inherited cardiomyopathy 

 

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TruSight Cardiomyopathy
 
TG-141-1004
 
 
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TruSight Inherited Disease

 

Focusing on severe, recessive pediatric onset diseases.

  • Developed in collaboration with Dr. Stephen Kingsmore and team at Children's Mercy Hospital (CMH) for Pediatric Genomic Medicine, Dr. Carol Saunders at CMH, and Dr. Hilger Ropers at the Max Planck Institute
  • Targets 552 genes, including coding exons, intron-exon boundaries, and regions known to harbor pathogenic mutations 

 

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TruSight Inherited Disease
 
TG-141-1005
 
 
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TruSight Myeloid Sequencing Panel

 

The TruSight Myeloid Sequencing Panel uses expert-defined content and proven next-generation sequencing (NGS) technology to identify somatic mutations in myeloid malignancies.

 

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TruSight Myeloid Sequencing Panel
 
FC-130-1010
 
 
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TruSight Rapid Capture

 

Prepare target sequencing libraries 70% faster.

TruSight Rapid Capture are all-in-one kits for library preparation and enrichment that allow researchers to prepare TruSight target sequencing libraries 70% faster than any other method. TruSight Rapid Capture allows researchers to maximize the productivity of their lab personnel and Illumina sequencing technology, enabling library preparation and enrichment-based sequence capture for up to 96 samples in only 1.5 days.

 

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TruSight Rapid Capture
 
FC-140-1101
 
 
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TruSight Tumor

 

Access a deeper view of variation in solid tumors.

The TruSight Tumor sequencing panel takes a deeper view of variation in solid tumors including lung, colon, melanoma, gastric and ovarian. This enables clinical researchers to identify low-frequency variation across 26 genes for a more comprehensive view of somatic variation.

 

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TruSight Tumor
 
FC-130-2001
 
 
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TruSeq Custom Amplicon v1.5

 

TruSeq Custom Amplicon is a fully customizable, amplicon-based assay for targeted resequencing. With targeted resequencing, researchers can focus interrogation on key regions of genomic interest. The assay allows researchers to sequence up to 1536 amplicons in a single reaction using a simple workflow. This highly targeted approach offers unparalled efficiency for discovering, validating, and screening genetic variants in areas of research focus.

 

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TruSeq Custom Amplicon v1.5
 
FC-130-1001
 
 
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TruSeq Amplicon - Cancer Panel

 

The TruSeq Amplicon - Cancer Panel (TSACP) is a highly multiplexed targeted resequencing assay for detecting somatic mutations. Leveraging the long paired-end read capability, speed, and high data quality of the MiSeq system, entire projects can now be accomplished in days instead of months.

 

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TruSeq Amplicon - Cancer Panel
 
FC-130-1008
 
 
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TruSeq Targeted RNA Expression Kits

 

TruSeq Targeted RNA Expression Kits offer highly customizable mid- to high-plex gene expression profiling and validation for the MiSeq system. Overcoming significant challenges in cost and workflow, TruSeq Targeted RNA assays deliver a fully integrated solution, including convenient online panel design and ordering, a rapid and simple workflow starting with as little as 50 ng of RNA, and automated, on-instrument data analysis.

 

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TruSeq Targeted RNA Expression Kits
 
RT-101-1001
 
 
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Nextera Rapid Capture Exome and Expanded Exome Kits

 

The Nextera Rapid Capture Exome and Expanded Exome are all-in-one kits for sample preparation and exome enrichment that allow researchers to identify coding variants up to 70% faster than other methods. This optimized exome delivers 37 Mb of expertly selected exonic content and requires as little as 4 Gb of sequencing. An expanded kit increases coverage to UTRs and miRNA.

 

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Nextera Rapid Capture Exome and Expanded Exome Kits
 
FC-140-1000
 
 
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TruSeq Stranded Total RNA with Ribo-Zero Plant

 

TruSeq Stranded Total RNA Sample Preparation Kits with Ribo-Zero Plant deliver the clearest, most complete view of the plant transcriptome.

 

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TruSeq Stranded Total RNA with Ribo-Zero Plant
 
RS-122-2401
 
 
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PhiX Control v3

 

PhiX Control v3 is a reliable, adapter-ligated library used as a control for Illumina sequencing runs. The library is derived from the small, well-characterized PhiX genome, offering several benefits for sequencing and alignment.

 

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PhiX Control v3
 
FC-110-3001
 
 
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Nextera Rapid Capture Custom Enrichment Kit

 

Nextera Rapid Capture Custom Enrichment provides unparalleled access to genomic regions of interest. This custom assay allows researchers to sequence precious samples faster and more efficiently than ever before, using as little as 50 ng of input DNA. The kit offers add-on functionality to refine content over time, or add regions of unique interest to established panels like Nextera Rapid Capture Exome or TruSight content sets. Nextera Rapid Capture Custom Enrichment allows researchers to:

  • Generate ~0.5 – 15 Mbps of custom content in a single enrichment assay
  • Evolve a custom assay over time with add-on capability
  • Design a preferred assay using an intuitive, user-friendly, online tool

 

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Nextera Rapid Capture Custom Enrichment Kit
 
FC-140-1007
 
 
CUSTOMIZE

Nextera Mate Pair Sample Preparation Kit

 

Illumina's Nextera Mate Pair Sample Preparation Kit offers the only gel-free method for preparing up to 12 kb mate pair libraries with the industry’s lowest DNA input requirement. It is an ideal approach for de novo sequencing, genome finishing, and the detection of structural variation — even in the most complex genomes such as those derived from cancer.

  • Sequence precious samples when limited DNA is available
  • Prepare sequencing-ready libraries in less than two days
  • Generate highly diverse libraries with premier data quality

 

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Nextera Mate Pair Sample Preparation Kit
 
FC-132-1001
 
 
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TruSeq ChIP Sample Prep Kit

 

TruSeq ChIP Sample Preparation Kits provide a simple, cost-effective solution for generating chromatin immunoprecipitation sequencing (ChIP-Seq) libraries from ChIP-derived DNA. ChIP-Seq leverages next-generation sequencing (NGS) to quickly and efficiently determine the distribution and abundance of DNA-bound protein targets of interest across the genome. This method has become one of the most widely used NGS applications, enabling researchers to reliably and simultaneously identify binding sites of a broad range of targets across the entire genome, with high resolution and without constraints.

 

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TruSeq ChIP Sample Prep Kit
 
IP-202-1012
 
 
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TruSeq Targeted RNA Expression Apoptosis Panel

 

The TruSeq Targeted RNA Expression Apoptosis Panel is a predesigned gene-expression profiling solution for studying apoptosis, or programmed cell death. The panel includes assays targeting 117 commonly studied pro-apoptotic and anti-apoptotic genes. All TruSeq Targeted RNA fixed panels are experimentally validated to ensure strong correlation with RNA sequencing.

 

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TruSeq Targeted RNA Expression Apoptosis Panel
 
RT-201-1010
 
 
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TruSeq Targeted RNA Expression Cardiotoxicity Panel

 

The TruSeq Targeted RNA Expression Cardiotoxicity Panel is a predesigned gene-expression profiling solution for studying cellular pathways affected by cardiotoxic compounds or stress. The panel includes assays targeting 76 genes used as markers of the cardiotoxic response. All TruSeq Targeted RNA fixed panels are experimentally validated to ensure strong correlation with RNA sequencing.

 

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TruSeq Targeted RNA Expression Cardiotoxicity Panel
 
RT-201-1009
 
 
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TruSeq Targeted RNA Expression Cell Cycle Panel

 

The TruSeq Targeted RNA Expression Cell Cycle Panel is a predesigned gene-expression profiling solution for studying the cell cycle. The panel includes assays targeting 63 genes involved in all phases of the cell cycle as well as DNA replication. All TruSeq Targeted RNA fixed panels are experimentally validated to ensure strong correlation with RNA sequencing.

 

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TruSeq Targeted RNA Expression Cell Cycle Panel
 
RT-201-1003
 
 
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TruSeq Targeted RNA Expression Hedgehog Panel

 

The TruSeq Targeted RNA Expression Hedgehog Panel is a predesigned gene-expression profiling solution for studying hedgehog signaling. The panel includes assays targeting 76 genes involved in upstream and downstream signal transduction, as well as transcription factors and target genes. All TruSeq Targeted RNA fixed panels are experimentally validated to ensure strong correlation with RNA sequencing.

 

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TruSeq Targeted RNA Expression Hedgehog Panel
 
RT-201-1002
 
 
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TruSeq Targeted RNA Expression Neurodegeneration Panel

 

The TruSeq Targeted RNA Expression Neurodegeneration Panel is a predesigned gene-expression profiling solution for studying neurodegenerative and neurotoxic pathways such as those implicated in Alzheimer’s Disease. The panel includes assays targeting 77 genes involved in these pathways. All TruSeq Targeted RNA fixed panels are experimentally validated to ensure strong correlation with RNA sequencing.

 

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TruSeq Targeted RNA Expression Neurodegeneration Panel
 
RT-201-1001
 
 
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TruSeq Targeted RNA Expression NFκB Panel

 

The TruSeq Targeted RNA Expression NFκB Panel is a predesigned gene-expression profiling solution for studying NFκB signaling. The panel includes assays targeting 105 genes involved in upstream and downstream signal transduction, as well as NFκB transcription factors and target genes. All TruSeq Targeted RNA fixed panels are experimentally validated to ensure strong correlation with RNA sequencing.

 

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TruSeq Targeted RNA Expression NFκB Panel
 
RT-201-1008
 
 
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TruSeq Targeted RNA Expression p53 Panel

 

The TruSeq Targeted RNA Expression p53 Panel is a predesigned gene-expression profiling solution for studying p53 signaling. The panel includes assays targeting 52 genes involved in upstream and downstream signal transduction, as well as transcription factors and target genes. All TruSeq Targeted RNA fixed panels are experimentally validated to ensure strong correlation with RNA sequencing.

 

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TruSeq Targeted RNA Expression p53 Panel
 
RT-201-1007
 
 
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TruSeq Targeted RNA Expression Cytochrome p450 Panel

 

The TruSeq Targeted RNA Expression Cytochrome p450 Panel is a predesigned gene-expression profiling solution for studying Cytochrome p450 (CYP) genes. The panel includes assays targeting 28 cytochrome P450 genes involved in drug and toxin metabolism. All TruSeq Targeted RNA fixed panels are experimentally validated to ensure strong correlation with RNA sequencing.

 

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TruSeq Targeted RNA Expression Cytochrome p450 Panel
 
RT-201-1006
 
 
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TruSeq Targeted RNA Expression Stem Cell Panel

 

The TruSeq Targeted RNA Expression Stem Cell Panel is a predesigned gene-expression profiling solution for studying induction, differentiation and maintenance of a variety of stem cell types. The panel consists of assays targeting 100 genes, including stem cell markers, differentiation markers, pluripotency markers, cytokines and growth factors. All TruSeq Targeted RNA fixed panels are experimentally validated to ensure strong correlation with RNA sequencing.

 

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TruSeq Targeted RNA Expression Stem Cell Panel
 
RT-201-1005
 
 
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TruSeq Targeted RNA Expression Wnt Panel

 

The TruSeq Targeted RNA Expression Wnt Panel is a predesigned gene-expression profiling solution for studying Wnt signaling. The panel includes assays targeting 93 genes involved in upstream and downstream signal transduction, as well as transcription factors and target genes. All TruSeq Targeted RNA fixed panels are experimentally validated to ensure strong correlation with RNA sequencing.

 

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TruSeq Targeted RNA Expression Wnt Panel
 
RT-201-1004
 
 
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VeriSeq PGS

 

The VeriSeq PGS Kit takes advantage of next-generation sequencing (NGS) technology to provide comprehensive, accurate screening of all 24 chromosomes for selection of euploid embryos. Preimplantation genetic screening (PGS) results generated using VeriSeq PGS are comparable to those achieved with the widely used array-based 24sure technology. In addition, NGS offers the opportunity for improved assay workflow, higher throughput, and enhanced performance.

 

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VeriSeq PGS
 
RH-101-1001
 
 
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