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The HiSeq 2500 System features two run modes, rapid run and high output run mode, and the ability to process one or two flow cells simultaneously. This provides a flexible and scalable platform that supports the broadest range of applications and study sizes. Choose between rapid run and high output modes to fit your project needs. New high output reagents enable the generation of up to 1 terabase (Tb) of data, supporting the greatest number of samples per run while the new rapid run reagents enable reads up to 2x250 base pairs.
Rapid run mode provides quick results, allows efficient processing of a limited number of samples, and offers support of longer paired-end 250 base pair reads. The longer reads enable greater depth of coverage, improved assembly for de novo applications and aid in long read applications such as metagenomic analyses.
High output mode is ideal for larger studies or when the greatest depth of coverage is required. High output mode allows you to batch process > 6× more samples than rapid mode and to complete large projects quickly and efficiently. New HiSeq SBS Kits v4 produce 4 billion clusters per run, generating up to 1 Tb of data in 6 days.
|Rapid Run Mode||High Output Mode|
|TruSeq v3 Chemistry||HiSeq v4 Chemistry|
1 × 36 bp
2 × 50 bp
|Nextera Rapid Capture Exome
2 × 100 bp
|Human Whole Genome
|De novo Sequencing
2.5 Gb Genome
2 × 250 bp