HiSeq 2500 Offers Unprecedented Flexibility for Multiple Applications

The HiSeq 2500 System features two run modes, rapid run and high output run mode, and the ability to process one or two flow cells simultaneously. This provides a flexible and scalable platform that supports the broadest range of applications and study sizes. No other system lets you perform so many applications so quickly and easily. Choose between rapid run and high output modes offering scalable output to fit your project needs. New reagents enable the generation of up to 1 terabase (Tb) of data in high output mode, supporting the greatest number of samples per run.

Rapid run mode provides quick results

Rapid run mode provides quick results, allows efficient processing of a limited number of samples, and offers support of longer paired-end 150 base pair reads, which enable greater depth of coverage and improved assembly for de novo applications.

High output mode is perfect for larger studies

High output mode is ideal for larger studies or when the greatest depth of coverage is required. High output mode allows you to batch process > 6× more samples than rapid mode and to complete large projects quickly and efficiently. New HiSeq SBS Kits v4 produce 4 billion clusters per run, generating up to 1 Tb of data in 6 days.

  Rapid Run Mode High Output Mode
    TruSeq v3 Chemistry HiSeq v4 Chemistry
Transcription Factor
15M Reads
1 × 36 bp
40 samples
7 hours
200 samples
2 days
260 samples
29 hrs
40M Reads
2 × 50 bp
15 samples
16 hours
120 samples
5 days
100 samples
2.5 days
Nextera Rapid Capture Exome
100x Coverage
2 × 100 bp
20 samples
27 hours
115 samples
10 days
150 samples
5 days
Human Whole Genome
>30x coverage
1 sample
27 hours
6 samples
11 days
10 samples
6 days
De novo Sequencing
1.5 Gb Genome
100x Coverage
2 × 150 bp
1 sample
40 hours


Compare Illumina Sequencing System Specifications »

HiSeq 2500 System